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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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miRNA-486-5p: signaling targets and role in non-malignant disease

Adrianna Douvris1,2, Jose Viñas1, Kevin D Burns3,4

  • 1Division of Nephrology, Department of Medicine and Kidney Research Centre, The Ottawa Hospital Research Institute, University of Ottawa, 1967 Riverside Dr., Rm. 535, Ottawa, ON, K1H 7W9, Canada.

Cellular and Molecular Life Sciences : CMLS
|June 22, 2022
PubMed

Insights

MicroRNAs (miRNAs), like miR-486-5p, regulate gene expression and are implicated in disease. This review explores miR-486-5p

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, with aberrant levels linked to human diseases.
  • miR-486-5p, a muscle-enriched miRNA, is abundant in plasma and extracellular vesicles, showing potential as a disease biomarker.
  • Its dysregulation is observed in various conditions, including kidney diseases.

Purpose of the Study:

  • To review the biogenesis and regulation of miR-486-5p.
  • To identify and discuss validated gene targets of miR-486-5p.
  • To examine the biological effects of miR-486-5p in non-malignant diseases.

Main Methods:

  • Literature review of pre-clinical and clinical studies.
  • Analysis of miRNA biogenesis and regulatory mechanisms.
  • Identification and validation of miR-486-5p gene targets and their associated signaling pathways.

Main Results:

  • miR-486-5p targets include PTEN, FoXO1, Smad1/2/4, IGF-1, MMP-19, Sp5, HAT1, and NFAT5.
  • Suppression of PTEN and FoXO1 by miR-486-5p activates PI3K/Akt signaling.
  • Inhibition of TGF-β and IGF-1 signaling by miR-486-5p targeting Smad1/2/4 and IGF-1, respectively.

Conclusions:

  • miR-486-5p plays a significant role in regulating cellular processes like proliferation, migration, angiogenesis, and apoptosis.
  • Validated targets of miR-486-5p provide insights into its function in non-malignant diseases.
  • Further research into miR-486-5p's biological effects can enhance its potential as a diagnostic and therapeutic target.
Keywords:
AngiogenesisApoptosisExtracellular vesiclesFibrosisIschemia reperfusion injurymicroRNA

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