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Light-Induced Patterning of Electroactive Bacterial Biofilms.

Fengjie Zhao1, Marko S Chavez1, Kyle L Naughton1

  • 1Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States.

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Summary

Researchers developed a new method to pattern conductive bacterial biofilms using light-controlled gene expression. This technique allows for precise control over living electronics, enabling tunable conductivity in bioelectronic devices.

Keywords:
Shewanella oneidensis MR-1biofilm patterningelectrochemical gatingextracellular electron transfer

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Area of Science:

  • Bioelectronics
  • Microbiology
  • Materials Science

Background:

  • Electroactive bacterial biofilms offer unique living biomaterial properties, merging biological functions with electronic capabilities.
  • Current limitations in controlling biofilm geometry on electrodes hinder the advancement of bioelectronic devices.
  • Shewanella oneidensis is a model organism for studying electroactive biofilms.

Purpose of the Study:

  • To develop a lithographic strategy for patterning conductive biofilms of Shewanella oneidensis.
  • To control biofilm formation using a blue light-induced genetic circuit regulating aggregation protein CdrAB expression.
  • To demonstrate tunable conductivity and quantify the intrinsic conductivity of patterned biofilms.

Main Methods:

  • Utilized a blue light-induced genetic circuit to control CdrAB expression in Shewanella oneidensis.
  • Employed a lithographic approach for precise patterning of biofilms on transparent electrode surfaces.
  • Performed electrochemical measurements to assess biofilm conductivity and pattern size dependence.

Main Results:

  • Successfully patterned conductive Shewanella oneidensis biofilms on transparent electrodes.
  • Demonstrated tunable electrical conduction based on biofilm pattern size.
  • Quantified the intrinsic conductivity of the living biofilms, confirming theoretical predictions.
  • Validated a collision-exchange electron transport mechanism through experimental data.

Conclusions:

  • Developed a facile technique for controlled electroactive biofilm formation on electrodes.
  • The method enables precise spatial control over living electronics.
  • Findings have significant implications for the study and application of bioelectronics.
  • This work advances the field of living biomaterials and bioelectronic interfaces.