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NMT1 Enhances the Stemness of NSCLC Cells by Activating the PI3K/AKT Pathway

Wailong Zou1, Xinjun Zhang1, Yumin Wang1

  • 1Department of Respiratory and Critical Care Medicine, Aerospace Center Hospital, Beijing, China.

Pharmacology
|June 22, 2022
PubMed
Abstract

Insights

N-myristoyltransferase-1 (NMT1) promotes non-small cell lung cancer (NSCLC) stemness and metastasis by activating the PI3K/AKT pathway. NMT1 may serve as a therapeutic biomarker for NSCLC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Proteins play crucial roles as tumor promoters or suppressors in cancer progression.
  • N-myristoyltransferase-1 (NMT1) is a protein whose regulatory function in non-small cell lung cancer (NSCLC) warrants investigation.

Purpose of the Study:

  • To investigate the regulatory role of NMT1 in non-small cell lung cancer (NSCLC).
  • To elucidate the underlying molecular mechanisms by which NMT1 influences NSCLC progression.

Main Methods:

  • Assessed self-renewal using spheroid-formation assays.
  • Examined tumorigenic abilities in nude mice.
  • Measured protein and mRNA expression via Western blot, IHC, and RT-qPCR.
  • Evaluated cell migration, invasion, and drug resistance (IC50) using transwell and CCK-8 assays.

Main Results:

  • NMT1 expression was elevated in NSCLC spheroid cells and patient tumor tissues.
  • NMT1 enhanced NSCLC stemness, accelerated tumor metastasis, and increased cisplatin resistance.
  • NMT1 activation of the PI3K/AKT pathway was identified as a key mechanism driving stemness.
  • Higher NMT1 expression correlated with poorer survival rates in NSCLC patients.

Conclusions:

  • NMT1 significantly enhances NSCLC cell stemness through PI3K/AKT pathway activation.
  • NMT1 presents potential as a therapeutic biomarker for non-small cell lung cancer.
Keywords:
NMT1Non-small cell lung cancerPI3K/AKT pathwayStemness

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