Related Experiment Video

Updated: Sep 7, 2025

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

8.7K

PA0575 (RmcA) interacts with other c-di-GMP metabolizing proteins in Pseudomonas aeruginosa PAO1

Yanxiang Yao1, Naren Xi1, E Hai1

  • 1School of Basic Medicine, Department of Biochemistry and Molecular Biology, Ningxia Medical University.

The Journal of General and Applied Microbiology
|June 22, 2022
PubMed

Insights

Bis-(3,5)-cyclic diguanosine monophosphate (c-di-GMP) signaling is crucial in bacteria. This study found that Pseudomonas aeruginosa PA0575 (RmcA) interacts with MorA (PA4601) and FimX (PA4959), revealing complex c-di-GMP protein networks.

Area of Science:

  • Bacteriology
  • Molecular Biology
  • Biochemistry

Background:

  • Bis-(3,5)-cyclic diguanosine monophosphate (c-di-GMP) is a key bacterial second messenger regulating diverse physiological processes.
  • Pseudomonas aeruginosa possesses a complex system of genes involved in c-di-GMP metabolism, with emerging evidence of intricate protein interactions.

Purpose of the Study:

  • To investigate the interaction network of c-di-GMP metabolizing proteins in Pseudomonas aeruginosa PAO1.
  • To identify novel protein-protein interactions within the c-di-GMP signaling pathway.

Main Methods:

  • Bacteria-2-Hybrid (B2H) assay was employed to detect protein-protein interactions.
  • Focus on identifying interactions involving GGDEF-EAL dual domain proteins.

Main Results:

  • The GGDEF-EAL dual protein PA0575 (RmcA) was found to interact with two other dual proteins: PA4601 (MorA) and PA4959 (FimX).
  • These interactions highlight the complexity of the c-di-GMP metabolizing protein network in P. aeruginosa.

Conclusions:

  • The identified interactions contribute to a deeper understanding of the intricate c-di-GMP signaling network in bacteria.
  • This study provides foundational data for further research into the regulation of bacterial physiology by c-di-GMP.
Keywords:
P aeruginosa PAO1PA0575 (RmcA)PA4601 (MorA)PA4959 (FimX)c-di-GMP

More Related Videos

Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins12:11

Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins

Published on: March 29, 2015

11.7K
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay DRaCALA09:26

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay DRaCALA

Published on: March 19, 2021

3.6K

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
90
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.9K
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
50