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Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: Sep 7, 2025

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Imaging MS Analysis in Catharanthus roseus.

Kotaro Yamamoto^1,2,3, Katsutoshi Takahashi⁴, Sarah E O'Connor⁵

¹Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan. yamamoto.kot.qa@yokohama-cu.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|June 22, 2022

Summary

Plant metabolism regulation requires knowing metabolite locations. Imaging Mass Spectrometry (IMS) maps where secondary metabolites, like alkaloids, are found in Madagascar periwinkle (Catharanthus roseus) tissues.

Keywords:

Alkaloid Apocynaceae Catharanthus roseus Idioblast cell Imaging MS Laticifer cell Single-cell metabolomics Specialized metabolism

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Last Updated: Sep 7, 2025

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

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Published on: October 2, 2016

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

Area of Science:

Plant biology
Metabolomics
Biochemistry

Background:

Understanding plant metabolic regulation is crucial.
Metabolite localization within plant tissues and cells provides key insights.
Single-cell omics technologies have advanced plant gene discovery for metabolic enzymes and transporters.

Purpose of the Study:

To determine the spatial localization of secondary metabolites, particularly alkaloids.
To map metabolite distribution within the stem and leaf tissues of Catharanthus roseus.
To apply advanced imaging techniques for metabolite profiling.

Main Methods:

Utilized Imaging Mass Spectrometry (IMS) for spatial metabolomics.
Developed and applied methods to visualize metabolite distribution at the tissue level.
Focused on secondary metabolites, specifically alkaloids, in plant samples.

Main Results:

Successfully mapped the spatial distribution of secondary metabolites in Catharanthus roseus.
Identified specific locations of alkaloid accumulation within stem and leaf tissues.
Demonstrated the utility of IMS for plant metabolite localization studies.

Conclusions:

Imaging Mass Spectrometry is a powerful tool for mapping plant metabolite localization.
This study provides critical data on alkaloid distribution in Catharanthus roseus.
Understanding metabolite localization aids in dissecting plant metabolic pathways and regulation.