Sample Preparation, Data Acquisition, and Data Analysis for ¹⁵N-Labeled and Nonlabeled Monoterpene Indole Alkaloids...

Recent approaches developed in metabolomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) enabled us to assign a part of specialized metabolites in plants. However, the approaches are not good enough for the rest of the metabolites, which are still unknown. To characterize the unknown metabolites, more appropriate and precise approaches need to be developed. Here, a procedure to analyze ¹⁵N-labeled and nonlabeled LC-MS/MS data for identification of monoterpene indole alkaloids was developed.