Techniques to Study Automethylation of Histone Methyltransferases and its Functional Impact
Luis Popoca1,2, Chul-Hwan Lee3,4,5,6
1Department of Biochemistry and Molecular Pharmacology, NYU School of Medicine, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 22, 2022
Abstract:
The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.
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