Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

520
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
520
Centrifugation01:05

Centrifugation

2.5K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.5K
Dialysis01:15

Dialysis

795
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
795
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

6.1K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.1K
Chromatography: Introduction01:10

Chromatography: Introduction

4.6K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
4.6K
Electrophoresis: Overview01:20

Electrophoresis: Overview

2.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of Proteins and Peptides by Electrokinetic Stacking Coupled with Paper Spray Mass Spectrometry.

Analytical chemistry·2026
Same author

Efficient, Low-Cost, and High-Throughput Sodium Dodecyl Sulfate (SDS) Removal from Protein Digests Using Weak-Anion Exchange.

Journal of the American Society for Mass Spectrometry·2024
Same author

Addressing Challenges in Ion-Selectivity Characterization in Nanopores.

Journal of the American Chemical Society·2024
Same author

In-Membrane Enrichment and Peptic Digestion to Facilitate Analysis of Monoclonal Antibody Glycosylation.

Analytical chemistry·2024
Same author

Rapid Quantitation of Various Therapeutic Monoclonal Antibodies Using Membranes with Fc-Specific Ligands.

Analytical chemistry·2023
Same author

Analysis of Protein Glycosylation after Rapid Digestion Using Protease-Containing Membranes in Spin Columns.

Journal of the American Society for Mass Spectrometry·2023

Related Experiment Video

Updated: Sep 7, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

24.7K

Ion Separations Based on Spontaneously Arising Streaming Potentials in Rotating Isoporous Membranes.

Chao Tang1, Andriy Yaroshchuk2,3, Merlin L Bruening1,4

  • 1Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46656, USA.

Membranes
|June 23, 2022
PubMed
Summary

Highly selective ion separations using charged membranes can be improved by controlling experimental conditions. Optimizing membrane rotation and feed composition significantly enhances lithium and potassium ion separation efficiency.

Keywords:
ion separationsselectivitystreaming potential

More Related Videos

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

8.6K
Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
07:45

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Published on: August 16, 2018

10.1K

Related Experiment Videos

Last Updated: Sep 7, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

24.7K
Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

8.6K
Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
07:45

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Published on: August 16, 2018

10.1K

Area of Science:

  • Membrane science
  • Separation technology
  • Electrochemistry

Background:

  • Membrane-based ion separations offer an alternative to traditional methods.
  • Previous studies showed charged isoporous membranes can separate Li+ and K+.
  • Separation relies on streaming potentials and electromigration.

Purpose of the Study:

  • To investigate factors influencing highly selective ion separations.
  • To optimize conditions for enhanced Li+/K+ separation using charged membranes.
  • To understand the role of concentration polarization in separation efficiency.

Main Methods:

  • Utilizing pressure-driven flow through negatively charged isoporous membranes.
  • Employing a rotating membrane system to control concentration polarization.
  • Varying transmembrane pressure, ionic strength, feed ratio, and rotation rate.

Main Results:

  • Achieved Li+/K+ selectivities up to 150 with optimized membrane rotation (1000 rpm).
  • Selectivity decreased significantly at lower rotation rates (1.3 at 95 rpm).
  • Increased ionic strength and feed ratio negatively impacted selectivity.

Conclusions:

  • Careful control of experimental conditions, especially concentration polarization, is crucial for high selectivity.
  • Membrane rotation is a key parameter for enhancing ion separation performance.
  • This technique shows promise for efficient production of pure salts.