Related Experiment Video

Updated: Sep 7, 2025

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

641

NMR-Based Metabolomics in Differential Diagnosis of Chronic Kidney Disease (CKD) Subtypes

Styliani A Chasapi1, Evdokia Karagkouni1, Dimitra Kalavrizioti2

  • 1Department of Pharmacy, University of Patras, 26504 Patras, Greece.

Metabolites
|June 23, 2022
PubMed

Insights

Early-stage Chronic Kidney Disease (CKD) urine metabolomics can help identify specific causes. Nuclear Magnetic Resonance (NMR) analysis distinguished IgA Nephropathy from other CKD types, aiding diagnosis.

Area of Science:

  • Nephrology
  • Metabolomics
  • Biochemistry

Background:

  • Chronic Kidney Disease (CKD) is a significant global health issue.
  • Early diagnosis and treatment are crucial for delaying CKD progression and kidney failure.
  • Understanding CKD etiology is vital for targeted therapeutic strategies.

Purpose of the Study:

  • To investigate if urine metabolomics can reflect the etiology of early-stage CKD.
  • To analyze the urine metabolic fingerprint of patients with common CKD types using NMR spectroscopy.
  • To differentiate between Chronic Glomerulonephritis, Diabetic Nephropathy (DN), and Hypertensive Nephrosclerosis (HN) based on urinary metabolic profiles.

Main Methods:

  • Collected urine samples from 108 CKD patients.
  • Utilized Nuclear Magnetic Resonance (NMR) spectroscopy for metabolomic analysis.
  • Compared metabolic profiles across different CKD etiologies and control groups.

Main Results:

  • Successfully clustered patients with Chronic Glomerulonephritis.
  • Identified a distinct urine metabolome for IgA Nephropathy, suggesting its unique origin.
  • Found no specific metabolic patterns for Diabetic Nephropathy (DN) and Hypertensive Nephrosclerosis (HN), aligning with their shared clinical presentation.
  • Characterized metabolic fluctuations between CKD subtypes and controls.

Conclusions:

  • Urine metabolomics shows promise for identifying the etiology of early-stage CKD, particularly IgA Nephropathy.
  • NMR-based metabolomics can aid in differentiating CKD subtypes.
  • Findings support improved diagnostic and prognostic approaches for CKD and offer insights into its pathology.

Keywords:
CKDIgANMR spectroscopyeGFRglomerulonephritismembranousmetabolomicsurine

More Related Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

278
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy07:54

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Published on: December 15, 2011

13.2K

Related Concept Videos

Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
77
Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
65
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
81
Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
3.4K
Chronic Kidney Disease IV: Nursing Management01:18

Chronic Kidney Disease IV: Nursing Management

Nursing management is essential for preventing complications, maintaining stability, and improving patients' quality of life in chronic kidney disease (CKD). By using a structured approach, nurses help slow CKD progression and support effective patient care​.1. Comprehensive patient assessmentEffective management begins with nurses reviewing the patient’s medical history, and identifying key risk factors like diabetes, hypertension, and nephrotoxic drug use. Nurses assess signs of...
50
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
56