Modulating the Activity of Enzyme in Metal-Organic Frameworks Using the Photothermal Effect of Ti₃C₂ Nanosheets

Enzymes are versatile catalysts with high potential in various applications, and much attention has been paid to the stability improvement of native enzymes and activity modulation. Encapsulation in metal-organic frameworks (MOFs) as an efficient strategy for protecting fragile native enzymes while modulating the activity of enzymes remotely, which is practically demanded, has rarely been explored in MOF-encapsulated enzymes. Herein, Ti₃C₂ nanosheets exhibiting photothermal effect and biocompatibility were encapsulated in Cyt c-embedded ZIF-8 to tailor the enzymatic activity remotely by near-infrared (NIR) irradiation for the first time. By exposure to NIR light, the temperature of an aqueous solution containing Ti₃C₂/Cyt c@ZIF-8 increases obviously (up to 15 °C), while that of Cyt c@ZIF-8 shows no change. The enzymatic activity in the composites with a certain amount of nanosheets increases, which is attributed to the created defect and transformed microenvironment caused by the introduction of nanosheets. Importantly, the enzymatic activity in ZIF-8 can be further enhanced up to 150% under NIR light irradiation, and this enhancement can be modulated flexibly by varying laser power density. Our investigations indicate that Ti₃C₂ nanosheets are promising candidates for modulating the activity of encapsulated enzymes remotely.