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CellTrace™ Violet Flow Cytometric Assay to Assess Cell Proliferation.
Marina B Lemieszek1, Scott D Findlay2,3, Gabrielle M Siegers4
1Department of Obstetrics and Gynecology, University of Alberta, Edmonton, AB, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2022
Summary
CellTrace™ Violet (CTV) is a reliable method for tracking cell proliferation and monitoring multiple cell divisions. This study details its application across various cell types and protocol optimization insights.
Area of Science:
- Cell Biology
- Immunology
- Biotechnology
Background:
- Cell proliferation is a fundamental biological process.
- Accurate tracking of cell division is crucial for understanding cellular dynamics.
- Existing methods may have limitations in sensitivity or multi-generational tracking.
Purpose of the Study:
- To describe the application of CellTrace™ Violet (CTV) for cell proliferation tracking.
- To demonstrate CTV's utility in diverse cell types.
- To share key observations from protocol optimization for CTV staining.
Main Methods:
- CellTrace™ Violet staining of cells.
- Monitoring fluorescence intensity reduction over cell divisions.
- Quantification of proliferation using flow cytometry.
- Application in various cell types and co-culture systems.
Main Results:
- CellTrace™ Violet permanently labels cellular proteins, enabling fluorescence tracking.
- Each cell division halves the fluorescence intensity per daughter cell.
- CTV allows for multi-generational proliferation monitoring.
- Successful application demonstrated across different cell types.
Conclusions:
- CellTrace™ Violet is a versatile and effective tool for quantifying cell proliferation.
- Protocol optimization is essential for reliable results in diverse cellular contexts.
- CTV facilitates the study of cell division dynamics and population tracking.
