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Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses
Albert Caballero-Solares1, Jennifer R Hall2, Xi Xue3
1Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL, Canada. acaballeroso@mun.ca.
Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2022
Abstract:
The reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is considered to be the gold standard for gene expression research. However, for this claim to be valid, RT-qPCR studies must test and optimize the quality of its RNA templates and assays. This chapter describes the experimental procedures required to generate reliable and reproducible gene expression results using RT-qPCR.
Keywords:
Gene of interestMIQE guidelinesNormalizer gene selectionPolymerase chain reactionPrimer pairsQuality testingRNAReverse transcriptioncDNA
