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Resveratrol inhibits HeLa cell proliferation by regulating mitochondrial function.

Yuming Zhang1, Fengyu Yuan2, Pei Li2

  • 1The International Centre for Precision Environmental Health and Governance, College of Life Sciences, Hebei University, Baoding 071002, China; Key Laboratory of Zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, China.

Ecotoxicology and Environmental Safety
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PubMed
Summary

Resveratrol (RES) inhibits HeLa cancer cell proliferation by disrupting mitochondrial function. This process involves reactive oxygen species (ROS) and autophagy, highlighting RES as a potential anti-cancer agent.

Keywords:
AutophagyCell homeostasisHeLa cellsMitochondrial functionResveratrol

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Cancer Research

Background:

  • Resveratrol (RES) shows promise in inhibiting cancer cell proliferation, but its precise mechanisms require further elucidation.
  • Mitochondrial function and dynamics are critical in cellular processes, including cancer progression.

Purpose of the Study:

  • To investigate the effects of resveratrol on HeLa cell proliferation.
  • To explore the role of mitochondrial metergasis and reactive oxygen species (ROS) in resveratrol-induced growth inhibition.

Main Methods:

  • HeLa cells were treated with varying concentrations of resveratrol for 30 minutes and 24 hours.
  • Assessed cell growth inhibition, ROS levels, LC3-II expression, mitochondrial membrane potential, mitochondrial DNA copy numbers, and respiratory capacities.

Main Results:

  • Resveratrol induced dose-dependent growth inhibition, ROS production, and LC3-II expression after 24 hours.
  • Short-term (30 min) low-dose RES decreased ROS and LC3-II while enhancing mitochondrial respiration.
  • Short-term high-dose RES triggered ROS overproduction, induced autophagy, and impaired mitochondrial function.

Conclusions:

  • Resveratrol inhibits HeLa cell proliferation by perturbing mitochondrial structure and function.
  • ROS-mediated autophagy plays a significant role in resveratrol's anti-cancer effects on HeLa cells.