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Related Experiment Videos

Micropipette holders: vented types are preferable to closed types.

J P Raynauld, J R Laviolette

    Journal of Neuroscience Methods
    |March 1, 1987
    PubMed
    Summary

    Temperature changes cause water-filled micropipettes to move and eject substances. This pipette motion impacts experiments in warm-blooded central nervous system (CNS) preparations.

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    Area of Science:

    • Neuroscience
    • Physiology
    • Biomedical Engineering

    Background:

    • Micropipette holders are crucial for delivering substances into biological tissues.
    • Water's significant volume expansion with temperature can lead to experimental artifacts.
    • Accurate delivery into the central nervous system (CNS) and spinal cord requires precise control.

    Purpose of the Study:

    • To identify the cause of pipette motion during CNS and spinal cord experiments.
    • To explain the ejection of electrolytes and marker dyes from micropipettes.
    • To highlight the impact of thermal expansion on experimental procedures.

    Main Methods:

    • Analysis of the coefficient of volume expansion for water.
    • Observation of micropipette behavior upon insertion into warm-blooded preparations.
    • Monitoring of pressure changes within closed micropipette systems.

    Main Results:

    • Large coefficient of volume expansion of water causes significant pipette motion.
    • Temperature increase upon insertion leads to pressure ejection of pipette contents.
    • This phenomenon affects the accuracy of substance delivery in CNS and spinal cord research.

    Conclusions:

    • Thermal expansion of water in micropipettes is a critical factor in experimental variability.
    • Understanding this effect is essential for improving the precision of neurophysiological experiments.
    • Strategies to mitigate pipette motion due to temperature changes are needed.

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