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Anti-obesity Effects of Dark Tea Extracts by Down-regulation of C/EBPα and PPARγ
Hyeong-Joon Lim1,2, Taek Joo Lim2, Jin Hyun Lee2
1College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
Background/Aim:
Dark tea, made by fermentation of tea leaves using microorganisms, is well known for its antiobesity effect; however, studies to identify this effect have not been sufficiently conducted. Herein, the anti-obesity effects of post-fermented dark tea were studied in high-fat diet mouse.
Materials And Methods:
Obesity was induced through a high-fat diet in C57BL/6 mice, and then dark tea extract powder (DTP) was orally administered daily for 12 weeks to evaluate the body and organ weights. Changes in the biochemical markers of obesity were evaluated to study the mechanism of the anti-obesity effects of DTP.
Results:
When DTP was administered to obesity mice, the weight and food intake reduced, blood aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) decreased, whereas high-density lipoprotein cholesterol (HDL-C) increased. Histopathology showed that steatosis and inflammation scores were reduced within the liver and adipocyte sizes were reduced within epididymal adipocyte. In addition, a significant decrease in blood insulin and hepatic TG and a significant increase in blood adiponectin were also confirmed. The results of western blot and qPCR in week 12, showed a significant decrease in the mRNA and protein levels of C/EBPα, and the mRNA levels of PPARγ in the liver.
Conclusion:
Dark tea extracts are thought to have an anti-obesity effect by reducing the levels of the main transcription factors that promote adipocyte differentiation, such as C/EBPα, and PPARγ. Therefore, diet products using dark tea extracts could be developed.
Insights
Post-fermented dark tea extract powder (DTP) reduced body weight and improved obesity markers in mice. This study suggests dark tea
Area of Science:
- Nutritional Science
- Biochemistry
- Pharmacology
Background:
- Dark tea, a fermented beverage, is recognized for potential anti-obesity properties.
- Previous research on dark tea's anti-obesity effects requires further investigation.
- Microbial fermentation in dark tea production may enhance its bioactive compounds.
Purpose of the Study:
- To investigate the anti-obesity effects of post-fermented dark tea extract powder (DTP).
- To elucidate the underlying mechanisms of DTP's anti-obesity action in a mouse model.
- To evaluate the impact of DTP on metabolic markers and gene expression related to adipogenesis.
Main Methods:
- Obesity was induced in C57BL/6 mice using a high-fat diet.
- Mice were orally administered DTP daily for 12 weeks.
- Body weight, organ weights, biochemical markers, and gene/protein expression were analyzed.
Main Results:
- DTP administration significantly reduced body weight and food intake.
- Key obesity markers including triglycerides, LDL-C, AST, and ALT levels decreased, while HDL-C increased.
- DTP downregulated key adipogenic transcription factors (C/EBPα and PPARγ) in the liver, reducing steatosis and adipocyte size.
Conclusions:
- Post-fermented dark tea extracts exhibit significant anti-obesity effects.
- DTP acts by inhibiting adipocyte differentiation through the downregulation of C/EBPα and PPARγ.
- Dark tea extracts show potential for development into functional foods or dietary supplements for weight management.
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