Toxicological effect assessment of aged biochar on Escherichia coli

Biochar (BC) is a biomass material that has a wide range of applications on the remediating heavy metals. In this experiment, we prepared BC (300 ºC, 500 ºC, and 700 ºC) and applied them to adsorb lead ions (Pb²⁺) to simulate BC treatment of Pb²⁺-contaminated soil. The retention capacity of BC for heavy metals was altered by means of bacterial culture, and the heavy metals released by BC can have toxicological effects on bacteria. This approach was used to assess the effects of long-term application of BC in heavily contaminated land with heavy metals on soil microorganisms. The results show that Escherichia coli survived in the medium containing lower doses of Pb²⁺-aged BC prepared at 300 ºC and 500 ºC (25 mg/L and 50 mg/L), depending on its ability of tolerating a certain amount of Pb²⁺. The addition of 100 mg/L Pb²⁺-aged BC prepared at 700 ºC not only significantly inhibited the growth of E. coli, but also promoted the release of citric acid from E. coli, which in turn triggered BC releasing more Pb²⁺. It is hoped that this will provide foundation to support the long-term application of BC in the remediation of heavy metal contaminated soils.