Cybrids and heterokaryons PEG-induced between D. discoideum amoebae and mouse fibroblasts

Optimal conditions for fusion of Dictyostelium discoideum amoebae with mouse fibroblasts using polyethylene glycol (PEG) are described. Three different kinds of experiments were done to look for the ways to obtain new cell lines heterokaryons or cybrids. In all cases, the final products of the fusion were cells showing new features, still viable for at least one week. To detect the cell membrane fusion, samples were observed by scanning electron microscope (SEM).