Intestinal Microecology of Mice Exposed to TiO₂ Nanoparticles and Bisphenol A

Exposure to titanium dioxide nanoparticles (TiO₂ NPs) and bisphenol A (BPA) is ubiquitous, especially through dietary and other environmental pathways. In the present study, adult C57BL/6J mice were exposed to TiO₂ NPs (100 mg/kg), BPA (0, 5, and 50 mg/kg), or their binary mixtures for 13 weeks. The 16S rDNA amplification sequence analysis revealed that co-exposure to TiO₂ NPs and BPA altered the intestinal microbiota; however, this alteration was mainly caused by TiO₂ NPs. Faecal metabolomics analysis revealed that 28 metabolites and 3 metabolic pathways were altered in the co-exposed group. This study is the first to reveal the combined effects of TiO₂ NPs and BPA on the mammalian gut microbial community and metabolism dynamics, which is of great value to human health. The coexistence of TiO₂ NPs and BPA in the gut poses a potential health risk due to their interaction with the gut microbiota.