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Comprehensive Analysis of hsa-miR-654-5p's Tumor-Suppressing Functions

Chuanyang Liu1, Lu Min1, Jingyu Kuang1

  • 1Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha 410073, China.

International Journal of Molecular Sciences
|June 24, 2022
PubMed

Insights

This study reveals microRNA-654-5p (miR-654-5p) acts as a tumor suppressor in lung adenocarcinoma (LUAD). Elevated miR-654-5p inhibits cancer cell growth, migration, and epithelial-mesenchymal transition, offering prognostic value.

Area of Science:

  • Molecular Oncology
  • Cancer Genomics
  • Biomarker Discovery

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development, metastasis, and patient prognosis.
  • The specific functions of hsa-miR-654-5p in lung cancer remain largely underexplored.
  • Understanding miRNA roles is vital for advancing cancer diagnostics and therapeutics.

Purpose of the Study:

  • To investigate the biological functions and regulatory patterns of hsa-miR-654-5p in lung cancer.
  • To identify potential targets and pathways regulated by miR-654-5p.
  • To evaluate the prognostic significance of miR-654-5p in lung adenocarcinoma (LUAD).

Main Methods:

  • Integrated bioinformatics analyses (functional, target prediction, ssGSEA) with in vitro experimental validation.
  • Identified miRNA-mRNA interactions and constructed protein-protein interaction networks.
  • Developed a five-gene risk scoring model for prognostic assessment.

Main Results:

  • miR-654-5p was significantly upregulated in LUAD tissues and correlated with patient survival.
  • Identified 275 potential targets, validating the miR-654-5p-RNF8 axis.
  • Demonstrated miR-654-5p exhibits tumor-suppressive functions by inhibiting LUAD cell proliferation, migration, and epithelial-mesenchymal transition (EMT).

Conclusions:

  • miR-654-5p acts as a tumor suppressor in lung cancer, inhibiting key oncogenic processes.
  • miR-654-5p holds significant prognostic value for LUAD patients.
  • Provides a foundational reference map for miR-654-5p function across various cancers.
Keywords:
bioinformaticsepithelial-mesenchymal transitionmiR-654-5ppan-cancertarget genes

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