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Characterization of Phages YuuY, KaiHaiDragon, and OneinaGillian Isolated from Microbacterium foliorum

Uylae Kim1, Elizabeth S Paul1, Arturo Diaz1

  • 1Biology Department, La Sierra University, Riverside, CA 92505, USA.

International Journal of Molecular Sciences
|June 24, 2022
PubMed

Insights

Three novel bacteriophages were isolated from Microbacterium foliorum. Phage YuuY demonstrated a broad host range and enhanced thermal stability, offering potential for M. foliorum phage therapy applications.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriology

Background:

  • Microbacterium foliorum is a Gram-positive bacterium commonly found in organic matter.
  • Bacteriophages are viruses that infect bacteria and are being explored for therapeutic applications.

Purpose of the Study:

  • To isolate and characterize novel bacteriophages targeting Microbacterium foliorum.
  • To assess the host range, stability, and infection mechanisms of isolated phages.

Main Methods:

  • Isolation of lytic bacteriophages from M. foliorum.
  • Characterization of phage morphology, genome, pH and thermal stability, and life cycle.
  • Bioinformatics analysis for phage classification.

Main Results:

  • Three bacteriophages (KaiHaiDragon, OneinaGillian, YuuY) were isolated.
  • Phage YuuY exhibited a broad host range, infecting M. aerolatum.
  • All phages showed pH stability (5-11), with YuuY having higher thermal tolerance (up to 80°C).
  • Short latent period (90 min) and two-fold burst size were observed for all phages.
  • All phages utilize carbohydrate receptors for infection.

Conclusions:

  • The isolated bacteriophages, particularly YuuY, show promising characteristics for potential M. foliorum control.
  • Phage YuuY's broad host range and thermal stability make it a strong candidate for phage therapy development.
Keywords:
bacteriophagesbioinformaticsgenomicshost rangephage therapyphages

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