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Published on: November 30, 2016
Differential Expression of Tissular miRNA-155 in Pediatric Gastritis
Săsăran Maria Oana1, Bănescu Claudia2, Riza Anca Lelia3
1Department of Pediatrics III, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, Gheorghe Marinescu Street No 38, 540136 Targu Mures, Romania.
Background:
MicroRNA molecules, among them the intensely studied miRNA-155 (miR-155), are regarded as potential biomarkers of chronic gastric inflammation and premalignant lesion progression. However, literature data are scarce in terms of pediatric studies and in the evaluation of the predictive role of miRNA in early gastric inflammation. This study aims to assess the differential expression of miR-155 in relation to pediatric gastritis.
Methods:
The present research was conducted on 192 patients with chronic dyspeptic symptoms who underwent upper digestive endoscopy. Bioptic samples were harvested for histopathological analysis and tissue miR-155 depiction. MiR-155 expression analysis was carried out through quantitative real-time polymerase chain reaction (qRT-PCR). The study population was divided into two groups: controls (93 patients) and study group (99 patients) with inflammatory modifications.
Results:
MiR-155 expression was augmented in patients with gastritis but did not differ significantly from controls (p = 0.16). An increase in miR-155 expression was noted in relation to chronic gastritis, H. pylori infection, or increase in gastritis severity, but these variations were not important (p = 0.30, p = 0.44, and p = 0.45, respectively).
Conclusions:
According to our study, pediatric gastritis increases, but does not greatly influence, miR-155 expression. Dynamic evaluation of miR-155 might enlighten its prognostic role in pediatric gastritis.
Insights
Pediatric gastritis slightly increases microRNA-155 (miR-155) expression but does not significantly influence it. Further dynamic evaluation of miR-155 may clarify its prognostic role in early gastric inflammation.
Area of Science:
- Gastroenterology
- Molecular Biology
- Pediatrics
Background:
- MicroRNAs, including miR-155, are potential biomarkers for gastric inflammation and premalignant lesions.
- Limited pediatric studies exist on miRNA's predictive role in early gastric inflammation.
- This study addresses the differential expression of miR-155 in pediatric gastritis.
Purpose of the Study:
- To assess the differential expression of miR-155 in pediatric gastritis.
- To investigate the relationship between miR-155 levels and early gastric inflammation in children.
- To evaluate the potential of miR-155 as a biomarker in pediatric gastric conditions.
Main Methods:
- 192 pediatric patients with dyspeptic symptoms underwent endoscopy and biopsy.
- Histopathological analysis and tissue miR-155 quantification via qRT-PCR were performed.
- Patients were categorized into controls (93) and a study group with inflammatory changes (99).
Main Results:
- MiR-155 expression was elevated in pediatric gastritis patients but not significantly different from controls (p=0.16).
- No significant variations in miR-155 were observed concerning chronic gastritis, H. pylori infection, or gastritis severity (p>0.30).
Conclusions:
- Pediatric gastritis shows a slight increase in miR-155 expression, but its influence is not substantial.
- Dynamic assessment of miR-155 could elucidate its prognostic value in pediatric gastritis.
- Further research is needed to fully understand miR-155's role in pediatric gastric health.
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