Related Experiment Video

Updated: Sep 6, 2025

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

12.4K

The Therapeutic Role of PNU-74654 in Hepatocellular Carcinoma May Involve Suppression of NF-κB Signaling

Min-You Wu1, Chi-Chih Wang1,2, Ya-Chuan Chang1

  • 1School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan.

Medicina (Kaunas, Lithuania)
|June 24, 2022
PubMed

Insights

PNU-74654, a Wnt/β-catenin inhibitor, demonstrates antitumor effects by reducing hepatocellular carcinoma cell viability and migration. Its mechanism involves inducing apoptosis and interfering with cell cycle regulation via the NF-κB pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Hepatocellular carcinoma (HCC) remains a significant global health challenge.
  • PNU-74654, a Wnt/β-catenin inhibitor, has shown prior antitumor activities.
  • The therapeutic potential of PNU-74654 in HCC requires detailed investigation.

Purpose of the Study:

  • To evaluate the cytotoxic effects of PNU-74654 on HCC cell lines.
  • To elucidate the molecular mechanisms underlying PNU-74654's action in HCC.

Main Methods:

  • Utilized HepG2 and Huh7 liver cancer cell lines.
  • Assessed cell viability, apoptosis (flow cytometry, Hoechst staining), and migration (wound healing assay).
  • Employed oncology arrays and western blotting to explore molecular pathways and protein expression.

Main Results:

  • PNU-74654 significantly decreased cell viability and inhibited cell migration.
  • Induction of apoptosis was observed, evidenced by an increased sub-G1 cell population and nuclear morphological changes.
  • Oncology array identified suppressed Bcl-xL and survivin expression; western blotting confirmed interference with cell cycle proteins via the NF-κB pathway.

Conclusions:

  • PNU-74654 exhibits antiproliferative and antimigration properties against HCC cells.
  • Antitumor activity is linked to cell cycle dysregulation and modulation of the NF-κB signaling pathway.
  • PNU-74654 presents a potential therapeutic agent for hepatocellular carcinoma.
Keywords:
NF-κBPNU-74654apoptosiscyclin Ahepatocellular carcinoma

More Related Videos

Y-90 Radioembolization and PD-1 Inhibitor as Neoadjuvant Treatment in Hepatocellular Carcinoma09:11

Y-90 Radioembolization and PD-1 Inhibitor as Neoadjuvant Treatment in Hepatocellular Carcinoma

Published on: May 24, 2024

588
Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

537

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.8K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K