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Optimized PCR-based Detection of Mycoplasma
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Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

Development of a template for use in immunofluorescent identification of mycoplasmas

V S Panangala, D H Lein

    Journal of Clinical Microbiology
    |April 1, 1978
    PubMed

    Abstract:

    A template for securing blocks of agar onto glass slides is described. Use of the template allows efficient handling as well as thorough washing of agar blocks during the immunofluorescence staining process.

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    Immunofluorescence Microscopy01:12

    Immunofluorescence Microscopy

    A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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