Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of the interaction between noise exposure and AGT gene polymorphisms in steelworkers on essential hypertension.

International archives of occupational and environmental health·2026
Same author

Next-generation therapies for osteoarthritis: the evolving role of cell therapy products.

Experimental & molecular medicine·2026
Same author

Pain levels and histological severity in a rat model of chemically-induced temporomandibular joint osteoarthritis.

BMC oral health·2026
Same author

Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric-Hydroxyapatite Composite Interference Screw.

Polymers·2026
Same author

Biomimetic Glycosaminoglycan-Enriched Electrospun Polymeric Scaffolds for Enhanced Early Tissue Regeneration.

Journal of functional biomaterials·2025
Same author

Three-Dimensional Models of the Dental Pulp: Bridging Fundamental Biology and Regenerative Therapy.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Sep 6, 2025

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.0K

Nano-Structured Ridged Micro-Filaments (≥100 µm Diameter) Produced Using a Single Step Strategy for Improved Bone

Nemeshwaree Behary1, Sandy Eap2, Aurélie Cayla1

  • 1ENSAIT, ULR 2461-GEMTEX-Génie et Matériaux Textiles, University of Lille, F-59000 Lille, France.

Molecules (Basel, Switzerland)
|June 24, 2022
PubMed
Summary

Researchers developed novel nanostructured poly-L-Lactic acid (PLLA) microfilaments for bone tissue engineering. These advanced scaffolds enhance human bone cell adhesion, proliferation, and osteogenic expression, offering a promising new material for regenerative medicine applications.

Keywords:
PLLAbone cell engineeringmelt-spinningmicrofilamentsnano-ridged fiber surfaceosteogenic expression

More Related Videos

Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

530
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

4.2K

Related Experiment Videos

Last Updated: Sep 6, 2025

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.0K
Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

530
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

4.2K

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Nanotechnology

Background:

  • Textile scaffolds are crucial for bone tissue engineering but often lack nanoscale features for optimal cell interaction.
  • Existing microfilaments (10–30 µm) are insufficient for robust osteoblast adhesion and spreading, requiring larger diameters (>30 µm).

Purpose of the Study:

  • To fabricate biodegradable, nanostructured poly-L-Lactic acid (PLLA) microfilaments with enhanced bone cell compatibility.
  • To investigate the potential of these nanostructured filaments as scaffolds for bone cell engineering.

Main Methods:

  • Single-step melt-spinning process to create PLLA microfilaments (100 µm and 230 µm) with inherent nano-scale ridges.
  • Atomic Force Microscopy (AFM) to characterize the nanostructure.
  • In vitro biological evaluation using human bone cells (MG 63) on woven scaffolds.

Main Results:

  • Successfully fabricated nanostructured PLLA microfilaments with ridge-like topographical features.
  • Demonstrated significantly enhanced human bone cell (MG 63) adhesion and proliferation compared to smooth fibers.
  • Observed elongated filopodia anchoring to nanostructures and confirmed in vitro osteogenic expression (osteocalcin, bone sialoprotein) after 21 days.

Conclusions:

  • The novel nanostructured PLLA microfilaments represent a new generation of biomaterials for bone tissue engineering scaffolds.
  • The integrated nanoscale topography promotes superior osteoblast response, including cell adhesion, proliferation, and differentiation.
  • These filaments offer a versatile platform for creating 2D/3D scaffolds with improved biological performance for bone regeneration.