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Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
Published on: June 9, 2023
Screening of Specific and Common Pathways in Breast Cancer Cell Lines MCF-7 and MDA-MB-231 Treated with
Keng-Shiang Huang1, Yi-Ting Wang2, Omkar Byadgi3
1The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, No. 8, Yida Rd., Jiaosu Village Yanchao District, Kaohsiung City 82445, Taiwan.
Abstract:
Our previous findings have shown that the chlorophyllides composites have anticancer activities to breast cancer cell lines (MCF-7 and MDA-MB-231). In the present study, microarray gene expression profiling was utilized to investigate the chlorophyllides anticancer mechanism on the breast cancer cells lines. Results showed that chlorophyllides composites induced upregulation of 43 and 56 differentially expressed genes (DEG) in MCF-7 and MDA-MB-231 cells, respectively. In both cell lines, chlorophyllides composites modulated the expression of annexin A4 (ANXA4), chemokine C-C motif receptor 1 (CCR1), stromal interaction molecule 2 (STIM2), ethanolamine kinase 1 (ETNK1) and member of RAS oncogene family (RAP2B). Further, the KEGG annotation revealed that chlorophyllides composites modulated DEGs that are associated with the endocrine system in MCF-7 cells and with the nervous system in MDA-MB-231 cells, respectively. The expression levels of 9 genes were validated by quantitative reverse transcription PCR (RT-qPCR). The expression of CCR1, STIM2, ETNK1, MAGl1 and TOP2A were upregulated in both chlorophyllides composites treated-MCF-7 and MDA-MB-231 cells. The different expression of NLRC5, SLC7A7 and PKN1 provided valuable information for future investigation and development of novel cancer therapy.
Insights
Chlorophyllides composites show anticancer effects on breast cancer cells by altering gene expression. Key genes like CCR1 and STIM2 were upregulated, suggesting potential for new cancer therapies.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Previous research indicated anticancer properties of chlorophyllides against breast cancer cell lines (MCF-7 and MDA-MB-231).
- Understanding the molecular mechanisms underlying chlorophyllides' anticancer activity is crucial for therapeutic development.
Purpose of the Study:
- To investigate the anticancer mechanism of chlorophyllides composites on breast cancer cell lines using microarray gene expression profiling.
- To identify differentially expressed genes (DEGs) and associated pathways modulated by chlorophyllides.
Main Methods:
- Microarray gene expression profiling was employed to analyze gene expression changes in MCF-7 and MDA-MB-231 cells treated with chlorophyllides.
- KEGG pathway analysis was performed to identify enriched biological pathways.
- Quantitative reverse transcription PCR (RT-qPCR) was used to validate the expression levels of selected genes.
Main Results:
- Chlorophyllides composites induced significant upregulation of 43 DEGs in MCF-7 and 56 DEGs in MDA-MB-231 cells.
- Commonly modulated genes included annexin A4 (ANXA4), chemokine C-C motif receptor 1 (CCR1), stromal interaction molecule 2 (STIM2), ethanolamine kinase 1 (ETNK1), and member of RAS oncogene family (RAP2B).
- KEGG analysis revealed pathway modulation related to the endocrine system in MCF-7 cells and the nervous system in MDA-MB-231 cells. Validation confirmed upregulation of CCR1, STIM2, ETNK1, MAGl1, and TOP2A.
Conclusions:
- Chlorophyllides composites exert anticancer effects through modulation of specific gene expression profiles in breast cancer cells.
- The identified DEGs and pathways provide insights into the anticancer mechanisms of chlorophyllides.
- Further investigation into genes like NLRC5, SLC7A7, and PKN1 may lead to novel cancer therapeutic strategies.
