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Impact of the Structural Modifications of Potato Protein in the Digestibility Process under Semi-Dynamic Simulated
Luis Jiménez-Munoz1, Emmanouil D Tsochatzis1,2, Milena Corredig1
1Department of Food Science, CiFOOD Center for Innovative Foods, Aarhus University, Agro Food Park 48, 8200 Aarhus, Denmark.
Food matrix structure significantly impacts potato protein digestibility. Heat treatment enhances protein breakdown and alters gastric emptying, influencing nutrient availability for plant-based diets.
Area of Science:
- Food Science
- Protein Chemistry
- Nutritional Science
Background:
- Rising consumer demand for plant-derived proteins necessitates understanding alternative protein ingredient functionality.
- Potato protein offers a nutritionally valuable amino acid profile and high technological functionality.
Purpose of the Study:
- To evaluate how structural differences in potato protein matrices affect in vitro digestibility.
- To investigate the impact of heat treatment on protein breakdown and gastric fate.
Main Methods:
- Preparation of four isocaloric potato protein matrices (suspension, gel, foam, heat-set foam).
- Utilizing a semi-dynamic in vitro digestion model to simulate gastrointestinal fate.
- Analysis of microstructure, protein breakdown via electrophoresis, and free amino acids using LC-TQMS.
Main Results:
- Heat-treated samples exhibited higher hydrolysis and lower trypsin inhibitory activity.
- In vitro digestible indispensable amino acid score was 0.9, limited by sulfur amino acids/valine.
- Heated samples demonstrated a slower gastric emptying rate.
Conclusions:
- The food matrix structure significantly influences the digestibility and gastrointestinal fate of potato protein.
- Heat treatment positively affects protein hydrolysis and nutrient availability.
- Understanding matrix effects is crucial for optimizing plant-based protein ingredients.
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