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Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Published on: May 19, 2023
Development of a mitochondrial sirtuin 4 FRET assay based on its activity for removing 3-hydroxy-3-methylglutaryl
Yan Li1, Yonglong Zhao1, Zhuoxian Cao1
1State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Provincial Key Laboratory of Pharmaceutics, School of Pharmacy, School of Basic Medicine, Guizhou Medical University Guiyang 550004 China binhe@gmc.edu.cn +86 851 6908218 +86 137 65113985.
Abstract:
SIRT4 belongs to one of three mitochondrial sirtuins, which plays important roles in regulating many biological processes and has significant implications for treating several human diseases. However, the development of those small compounds that can modulate SIRT4 activities is limited because there is no efficient SIRT4 assay available for screening its modulators. Thanks to recent discoveries of several enzymatic activities and substrates for SIRT4, we have developed a FRET-based assay suitable for screening SIRT4 modulators based on its activity of removing 3-hydroxy-3-methylglutaryl (HMG) lysine modification, which could be further coupled with a secondary FRET-based assay for other sirtuins to identify SIRT4-specific inhibitors or activators.
