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circ-RANGAP1/MicroRNA-542-3p/Myosin Regulatory Light Chain Interacting Protein Axis Modulates the Osteosarcoma Cell

Jundong Sheng1, Jin Liu1, Junwang Du2

  • 1Department of Orthopedics, First People's Hospital of Tianshui, Tianshui, 741000 Gansu, China.

Applied Bionics and Biomechanics
|June 24, 2022
PubMed
Abstract

Insights

Circular RNA RANGAP1 (circRNA-RANGAP1) promotes osteosarcoma progression by targeting miR-542-3p to upregulate MYLIP. Silencing circRNA-RANGAP1 inhibits tumor growth, offering a potential therapeutic strategy for osteosarcoma.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with a poor prognosis.
  • Circular RNAs (circRNAs) play crucial roles in various cancers, including OS.
  • Understanding the molecular mechanisms underlying OS progression is vital for developing effective treatments.

Purpose of the Study:

  • To investigate the role of circRNA-RANGAP1 in osteosarcoma.
  • To explore the regulatory network involving circRNA-RANGAP1, miR-542-3p, and MYLIP in OS cells.
  • To determine the impact of this pathway on OS cell biological functions.

Main Methods:

  • Expression analysis of circRNA-RANGAP1, miR-542-3p, and MYLIP in OS tissues and cell lines using RT-qPCR.
  • Correlation analysis between circRNA-RANGAP1 expression and clinicopathological features/prognosis.
  • Functional studies in MG-63 cells involving circRNA-RANGAP1 silencing and miR-542-3p manipulation.
  • Investigation of the targeting relationship between circRNA-RANGAP1, miR-542-3p, and MYLIP.

Main Results:

  • circRNA-RANGAP1 was upregulated in OS tissues and associated with poor clinicopathology and prognosis.
  • Elevated circRNA-RANGAP1 expression was observed in male patients, those younger than 20, and patients with advanced OS.
  • Silencing circRNA-RANGAP1 or overexpressing miR-542-3p inhibited OS cell progression.
  • circRNA-RANGAP1 directly targets miR-542-3p, leading to increased MYLIP expression.

Conclusions:

  • circRNA-RANGAP1 promotes osteosarcoma progression by sponging miR-542-3p and upregulating MYLIP.
  • circRNA-RANGAP1 serves as a potential diagnostic biomarker and therapeutic target for osteosarcoma.

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