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Related Concept Videos

Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
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Updated: Sep 6, 2025

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
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X-ray Stain Localization with Near-Field Ptychographic Computed Tomography.

Kirsten Taphorn1,2, Madleen Busse1,2, Johannes Brantl1,2

  • 1Chair of Biomedical Physics, Department of Physics, School of Natural Sciences, Technical University of Munich, 85748, Garching, Germany.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 24, 2022
PubMed
Summary
This summary is machine-generated.

Near-field ptychographic X-ray computed tomography visualizes X-ray contrast agent distribution within cells. This technique reveals intracellular stain locations, advancing contrast agent development.

Keywords:
contrast agentsptychographic computed tomographyquantitative X-ray imaging

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Area of Science:

  • Medical Imaging
  • Cell Biology
  • Materials Science

Background:

  • X-ray contrast agents are crucial for medical imaging but their cellular-level distribution is often unknown.
  • Current methods lack the resolution and sensitivity to precisely locate contrast agent accumulation within cells.

Purpose of the Study:

  • To demonstrate a novel method for assessing X-ray contrast agent distribution at the intracellular level.
  • To enable quantitative analysis of stain concentrations within cellular structures.

Main Methods:

  • Quantitative near-field ptychographic X-ray computed tomography was employed.
  • Basis material decomposition was used to identify and quantify X-ray stain locations.
  • Murine kidney samples stained with iodine potassium iodide and eosin Y were analyzed.

Main Results:

  • The technique achieved resolution sufficient to identify intracellular structures.
  • Distribution of two different X-ray stains within cells was successfully assessed.
  • Quantitative nanoscopic stain concentrations correlated well with dual-energy micro computed tomography measurements.

Conclusions:

  • Near-field ptychographic X-ray computed tomography provides unprecedented intracellular resolution for X-ray stains.
  • This method advances the understanding and development of novel X-ray contrast agents.
  • The approach is applicable to a wide range of X-ray stains.