Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

4.7K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.7K
Simple Staining Technique01:24

Simple Staining Technique

751
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
751
Differential Staining Technique01:26

Differential Staining Technique

583
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
583
Special Staining Techniques01:13

Special Staining Techniques

286
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
286
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

11.8K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
11.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epstein-Barr virus orchestrates spatial reorganization and immunomodulation in the classic Hodgkin lymphoma tumor microenvironment.

Cell reports. Medicine·2026
Same author

Mirvetuximab soravtansine plus pembrolizumab in recurrent folate receptor alpha-positive uterine serous carcinoma: a phase II trial.

Nature communications·2026
Same author

Same-Slide Spatial Multi-Omics Integration with IN-DEPTH Reveals Tumor Virus-Linked Spatial Reorganization of the Tumor Microenvironment.

Cancer discovery·2026
Same author

Graph neural network modeling of spatial tumor-immune interactions identifies prognostic cellular niches in non‑small cell lung cancer.

NPJ precision oncology·2026
Same author

TTF-1 Expression in Lung Adenocarcinoma: Clinicopathologic, Genomic, and Immunophenotypic Correlates and Outcomes to Immunotherapy-Based Treatments and KRAS<sup>G12C</sup> Inhibitors.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer·2026
Same author

Multi-omics analysis reveals differential benefits of immunotherapy±chemotherapy based on detailed smoking history in advanced non-small cell lung cancer.

Journal for immunotherapy of cancer·2025
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Sep 6, 2025

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

25.3K

Cell Staining.

Scott J Rodig

    Cold Spring Harbor Protocols
    |June 24, 2022
    PubMed
    Summary
    This summary is machine-generated.

    Cell staining with labeled antibodies reveals antigen presence, location, and quantity in cells and tissues. This versatile technique aids in pathology and cell purification, with ongoing improvements enhancing sensitivity and quantitation.

    More Related Videos

    Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
    22:49

    Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

    Published on: December 13, 2012

    35.8K
    Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
    09:06

    Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

    Published on: February 20, 2019

    8.4K

    Related Experiment Videos

    Last Updated: Sep 6, 2025

    Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
    07:00

    Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

    Published on: June 24, 2020

    25.3K
    Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
    22:49

    Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

    Published on: December 13, 2012

    35.8K
    Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
    09:06

    Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

    Published on: February 20, 2019

    8.4K

    Area of Science:

    • Immunohistochemistry
    • Cell Biology
    • Pathology

    Background:

    • Labeled antibodies are crucial for detecting antigens in biological samples.
    • Cell staining provides insights into antigen presence and subcellular localization.
    • Double-labeling techniques allow for comparative analysis of antigen distribution.

    Purpose of the Study:

    • To detail cell staining protocols for antigen detection.
    • To discuss constraints, antibody selection, and troubleshooting for cell staining.
    • To highlight the versatility and applications of cell staining techniques.

    Main Methods:

    • Utilizing labeled antibodies for antigen detection.
    • Employing double-labeling for simultaneous antigen detection.
    • Integrating cell staining with histological and autoradiographic methods.

    Main Results:

    • Cell staining determines antigen presence and subcellular localization.
    • Allows for comparative analysis of antigen distribution using double-labeling.
    • Can identify infectious organisms, neoplastic cell progenitors, and inflammatory responses.

    Conclusions:

    • Cell staining is a versatile technique for antigen characterization.
    • It can detect low antigen molecule numbers and estimate concentration.
    • Advancements in technology are increasing the sensitivity and quantitation of cell staining.