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The nucleotide sequence of a cDNA clone encoding the wheat Em protein
Nucleic Acids Research
|April 24, 1987
Summary
Researchers sequenced a wheat embryo cDNA clone encoding the Em protein. This protein, rich in glycine, has a molecular weight of 9,900 daltons and is part of a gene family.
Area of Science:
- Molecular Biology
- Plant Science
- Genetics
Background:
- Developing wheat embryos express specific proteins crucial for their development.
- The Em protein's function and genetic basis were previously uncharacterized.
Purpose of the Study:
- To determine the nucleotide and amino acid sequence of the wheat Em protein.
- To investigate the genetic organization of the Em protein.
Main Methods:
- Isolation and sequencing of a cDNA clone from developing wheat embryos.
- Deduction of amino acid sequence from nucleotide sequence.
- Amino acid sequencing of purified Em protein fragments.
- Southern blot analysis to assess gene family size.
Main Results:
- The complete coding region and 3' non-translated flank of the Em protein were sequenced.
- The deduced amino acid sequence revealed high glycine content (18 mol%) and a molecular weight of approximately 9,900 daltons.
- Southern blot analysis indicated that Em is encoded by a gene family of about ten members.
Conclusions:
- The study provides the full sequence of the wheat Em protein and its genetic context.
- Em protein is encoded by a multi-gene family in wheat, suggesting potential functional redundancy or diversification.