Orrella daihaiensis sp. nov., a bacterium isolated from Daihai Lake in Inner Mongolia

A novel aerobic, Gram-staining-negative, non-motile, short-rod-shaped strain, designated f23^T, was obtained from Daihai Lake, Inner Mongolia, Republic of China. 16S rRNA gene sequences analysis showed that f23^T belongs to the genus Orrella and is most closely related to Orrella marina H-Z20^T with 98.35% sequence similarity. The strain was oxidase positive, catalase positive and had well growth at pH 6.5-8.5, at temperature 28-40 °C and at 0-4.5% (w/v) NaCl. Colonies incubated at 37 °C on marine 2216 agar for 3 days were white, smooth, transparent, circular and less than 1.0 mm in diameter. The total genome size of f23^T was 2,803,849 bp with a G + C content of 52.79%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain f23^T and O. marina H-Z20^T were 69.62% and 20.5%, which both below the species delineation threshold. Chemotaxonomic analysis showed that C_16:0, cyclo-C_17:0, C_18:0, Sum Feature 3 (C_16:1ω7c and/or C_16:1ω6c) and Sum Feature 8 (C_18:1ω6c and C_18:1ω7c) as the major fatty acids, ubiquinone-8 as the major isoprenoid quinone, phosphatidylethanolamine as the major cellular polar lipids. Based on the polyphasic analysis, f23^T represents a novel species within the genus Orrella, for which the name Orrella daihaiensis sp. nov. is proposed. The type strain is f23^T (= CGMCC 1.18761 ^T = KCTC 82425 ^T).