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High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

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Cell-repellent polyampholyte for conformal coating on microstructures.

Kohei Suzuki^1,2, Yoshiomi Hiroi², Natsuki Abe-Fukasawa²

¹Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa, 240-8501, Japan.

Scientific Reports

|June 25, 2022

Summary

Prevelex coatings prevent protein and cell adhesion on biomedical devices. This new polyampholyte material enables uniform spheroid formation and de novo hair follicle generation in tissue engineering.

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Last Updated: Sep 6, 2025

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Area of Science:

Biomaterials Science
Surface Chemistry
Tissue Engineering

Background:

Repellent coatings are essential for biomedical and analytical devices to prevent unwanted protein and cell adhesion.
Nonspecific adhesion can compromise device function and experimental results.

Purpose of the Study:

To synthesize and characterize prevelex, a novel polyampholyte coating material.
To evaluate prevelex coatings for microdevices used in cell culture and tissue engineering.

Main Methods:

Prevelex synthesis and characterization.
Dip-coating of prevelex onto hydrophobic substrates (polystyrene, polydimethylsiloxane).
Surface analysis using scanning electron microscopy.
Cell culture and spheroid formation in prevelex-coated microwell arrays.
In vivo transplantation of cell aggregates for hair follicle regeneration.

Main Results:

Prevelex coatings rendered hydrophobic surfaces highly hydrophilic and electrically neutral.
The coating process was effective within a moderate prebake temperature range (50-150 °C).
Scanning electron microscopy confirmed conformal, ultra-thin film formation on micro/nano structures.
Prevelex coatings outperformed other materials in facilitating uniform spheroid formation with various cell types.
Prevelex-coated microwell arrays supported the formation of hair follicle germ-like aggregates from mouse embryonic cells.
Transplantation of these aggregates successfully generated de novo hair follicles in nude mice.

Conclusions:

Prevelex offers a robust and effective approach for creating non-fouling surfaces.
This coating technology is suitable for advanced tissue engineering and biomedical applications.
Prevelex demonstrates significant potential for developing next-generation cell culture and regenerative medicine devices.