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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Genomic analysis of a novel active prophage of Hafnia paralvei

Lingting Pan1,2, Dengfeng Li3, Wei Lin2

  • 1Key Laboratory of Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China.

Archives of Virology
|June 25, 2022
PubMed

Insights

Researchers discovered a novel Hafnia phage, yong2, from Hafnia paralvei bacteria. Phylogenetic analysis suggests it represents a new class of prophages within the Hafniaceae and Enterobacteriaceae families.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Prophages within Hafniaceae bacteria are poorly understood.
  • Hafnia paralvei is a species within the Hafniaceae family.

Purpose of the Study:

  • To characterize a novel Hafnia phage, yong2, induced from Hafnia paralvei.
  • To investigate the phylogenetic relationships and evolutionary status of Hafnia phage yong2.

Main Methods:

  • Induction of phage yong2 from Hafnia paralvei using mitomycin C.
  • Morphological characterization of the phage particle.
  • Whole-genome sequencing and analysis of Hafnia phage yong2.
  • Phylogenetic analysis using conserved sequences.

Main Results:

  • Hafnia phage yong2 has an elliptical head (45 × 38 nm) and a long noncontractile tail (157 × 4 nm).
  • The complete genome is 39,546 bp double-stranded DNA with 59 open reading frames (ORFs) and a G+C content of 49.9%.
  • Phylogenetic analysis placed yong2 within a novel clade, suggesting a unique evolutionary status and conserved sequences among related prophages.

Conclusions:

  • Hafnia phage yong2 represents a newly identified phage with distinct genomic and phylogenetic characteristics.
  • The findings suggest the existence of a novel class of prophages in Hafniaceae and Enterobacteriaceae bacteria.
  • Further research is needed to explore the evolutionary significance and conserved sequences of this new prophage class.

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