Dual-band fluorescence detection of double-stranded DNA with QDs-Mn²⁺-pefloxacin

By integrating the fluorescence of quantum dots (QDs) and Mn²⁺-pefloxacin mesylate (Mn²⁺-pefloxacin), a new type of dual-band fluorescence biosensor for high-efficiency and sensitive determination of double-stranded DNA (dsDNA) is developed. The biosensor is based on the fluorescence "OFF-ON" mode of both QDs and QDs-Mn²⁺-pefloxacin. The Mn²⁺-pefloxacin complex can quench the QDs fluorescence via photoinduced electron transfer (PET), and its fluorescence is also quenched. Due to the specificity and strong binding affinity of dsDNA for the Mn²⁺-pefloxacin complex, it can break the low fluorescent QDs-Mn²⁺-pefloxacin and restore the fluorescence of QDs and Mn²⁺-pefloxacin complex in their respective bands. Therefore, the dual-band fluorescence quantitative detection of dsDNA by QDs-Mn²⁺-pefloxacin can be achieved, while bovine serum albumin, single-stranded DNA, and bio-related ions do not yield similar results. Furthermore, the possible reaction mechanisms are systematically discussed. The detection limits (3δ/K) of herring sperm (hs) DNA in the fluorescence recovery bands of QDs and Mn²⁺-pefloxacin complex are 0.0142 and 0.0465 μg/mL, respectively. The developed biosensor was used for dsDNA detection in synthetic samples, and desirable results are obtained.