Genomic Prediction Using LD-Based Haplotypes in Combined Pig Populations

The size of reference population is an important factor affecting genomic prediction. Thus, combining different populations in genomic prediction is an attractive way to improve prediction ability. However, combining multireference population roughly cannot increase the prediction accuracy as well as expected in pig. This may be due to different linkage disequilibrium (LD) pattern differences between population. In this study, we used the imputed whole-genome sequencing (WGS) data to construct LD-based haplotypes for genomic prediction in combined population to explore the impact of different single-nucleotide polymorphism (SNP) densities, variant representation (SNPs or haplotype alleles), and reference population size on the prediction accuracy for reproduction traits. Our results showed that genomic best linear unbiased prediction (GBLUP) using the WGS data can improve prediction accuracy in multi-population but not within-population. Not only the genomic prediction accuracy of the haplotype method using 80 K chip data in multi-population but also GBLUP for the multi-population (3.4-5.9%) was higher than that within-population (1.2-4.3%). More importantly, we have found that using the haplotype method based on the WGS data in multi-population has better genomic prediction performance, and our results showed that building haploblock in this scenario based on low LD threshold (r = 0.2-0.3) produced an optimal set of variables for reproduction traits in Yorkshire pig population. Our results suggested that whether the use of the haplotype method based on the chip data or GBLUP (individual SNP method) based on the WGS data were beneficial for genomic prediction in multi-population, while simultaneously combining the haplotype method and WGS data was a better strategy for multi-population genomic evaluation.