Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Levels of Use of a GIS01:29

Levels of Use of a GIS

97
Geographic Information Systems (GIS) operate across three levels of application, each representing an increasing degree of complexity: data management, analysis, and prediction. These levels reflect the expanding functionality and versatility of GIS technology in handling spatial data for diverse purposes.Data ManagementAt its foundational level, GIS serves as a tool for data management, enabling the input, storage, retrieval, and organization of spatial data. This level is often employed in...
97
Profile Leveling and Cross Sections01:26

Profile Leveling and Cross Sections

564
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes.  Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
564
Methods of Obtaining Topography01:25

Methods of Obtaining Topography

111
Topography involves measuring and mapping land elevations, natural features, and artificial structures to create accurate representations of the terrain. Topographic surveying relies on traditional and modern methods, each with distinct advantages and limitations.Traditional Surveying Methods:Transit stadia surveys and plane table surveys were widely used traditional surveying methods. These techniques relied on instruments like theodolites and stadia rods for measuring distances and angles,...
111
Manipulation and Analysis01:21

Manipulation and Analysis

58
GIS manipulation and analysis functions are vital for decision-making and planning. These activities range from data retrieval tasks, such as selecting information based on specific criteria, to advanced analytical techniques that address complex spatial problems.One critical GIS analysis method is overlaying, which combines multiple data layers to examine impacts. For example, overlaying a river-dammed lake boundary with road networks can identify affected infrastructure. Another common...
58
Design Example: Identifying the Locations of Monuments in the Field Using Global Positioning System Device01:30

Design Example: Identifying the Locations of Monuments in the Field Using Global Positioning System Device

166
Surveyors use Global Positioning System (GPS) technology to measure the precise location and elevation of points on Earth. In a recent survey, GPS receivers were used to determine the coordinates and elevations of two park monuments. The process involved careful mission planning, data collection, and correction to ensure accuracy. The survey began with mission planning to identify optimal satellite visibility and minimize Position Dilution of Precision (PDOP). A geodetic control point...
166
Gravimetry: Overview01:05

Gravimetry: Overview

6.6K
Gravimetric analysis is a quantitative method where the analyte is isolated and weighed directly or after conversion into a substance of known composition. Gravimetric analysis can be classified as precipitation, electrogravimetry, volatilization, and particulate gravimetry, based on the method used to isolate the analyte.
In precipitation gravimetry, the analyte is converted into a precipitate and weighed. For example, the silver content in a sample can be estimated by precipitating and...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI-based histopathology analysis predicts checkpoint inhibitor response in advanced melanoma and identifies patterns associated with response.

European journal of cancer (Oxford, England : 1990)·2026
Same author

Explainable machine learning identifies features and thresholds predictive of immunotherapy response.

Scientific reports·2026
Same author

Integrated multi-omics identifies distinct macrophage alterations during progression of metabolic dysfunction-associated steatohepatitis.

Nature genetics·2026
Same author

Local immunotherapy in the neoadjuvant treatment of cancer: optimizing efficacy while limiting toxicity?

Journal for immunotherapy of cancer·2026
Same author

Long-term cognitive function after treatment with immune checkpoint inhibition for melanoma.

Journal of cancer survivorship : research and practice·2026
Same author

From prediction to precision: Biomarker discovery and predictive modeling for personalized immune checkpoint blockade therapy.

European journal of cancer (Oxford, England : 1990)·2026
Same journal

Th17-mediated antitumor immunity in patient-derived organoid and autologous immune cell cocultures predicts response to immunotherapy in head and neck cancer.

Immuno-oncology technology·2026
Same journal

The genome takes the wheel: CAR T with built-in circuits.

Immuno-oncology technology·2026
Same journal

Combining circulating inflammatory proteins and thymidine kinase activity to predict survival in melanoma patients receiving immune checkpoint inhibitors.

Immuno-oncology technology·2026
Same journal

Fresh-blood immune profiling of immune-related adverse events in real-world patients identifies organ-specific and steroid responsiveness signatures.

Immuno-oncology technology·2026
Same journal

The effect of neoadjuvant radiotherapy on immune cell infiltrates in myxofibrosarcoma.

Immuno-oncology technology·2026
Same journal

Safety of intratumoral immunostimulatory LOAd703 gene therapy combined with chemotherapy in patients with advanced cancer.

Immuno-oncology technology·2026
See all related articles

Related Experiment Video

Updated: Sep 6, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K

A user's perspective on GeoMxTM digital spatial profiling.

Trieu My Van1, Christian U Blank1

  • 1Netherlands Cancer Institute, Amsterdam, The Netherlands.

Immuno-Oncology Technology
|June 27, 2022
PubMed
Summary
This summary is machine-generated.

New digital spatial profiling (DSP) technology allows detailed, simultaneous protein expression analysis in specific regions of formalin-fixed paraffin-embedded (FFPE) tissues. This aids in understanding tumor heterogeneity and identifying potential biomarkers for improved patient therapy.

Keywords:
DSPIHCMultiplexNanoStringRegion of interestTranslational research

More Related Videos

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.4K
Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios
06:02

Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios

Published on: October 6, 2020

2.4K

Related Experiment Videos

Last Updated: Sep 6, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.4K
Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios
06:02

Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios

Published on: October 6, 2020

2.4K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cancer Research

Background:

  • Multiplex immunohistochemistry has limitations for spatial protein expression analysis in heterogeneous tissues.
  • Detailed, simultaneous protein expression profiling within specific regions of interest (ROI) is needed.

Purpose of the Study:

  • To describe the experience with NanoString's GeoMx Digital Spatial Profiling (DSP) platform.
  • To provide an independent insight into the usability, advantages, and limitations of the DSP platform for potential users.

Main Methods:

  • Utilized GeoMx DSP technology on cancer FFPE tissues.
  • Employed probes tagged with photocleavable DNA oligos released via UV exposure in ROIs.
  • Quantified released oligos using NanoString's nCounter system for protein expression profiling.

Main Results:

  • The GeoMx DSP platform enables simultaneous, high-level multiplexing for protein expression profiling on FFPE tissues.
  • Digital quantification provides detailed expression profiles within discrete ROIs.
  • Experience gained from the beta version of the DSP platform.

Conclusions:

  • GeoMx DSP is a non-destructive technique for in-depth regional protein expression profiling.
  • DSP facilitates better characterization of tumor heterogeneity and micro-environment.
  • Potential for improved patient therapy and biomarker signature identification.