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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Related Experiment Video

Updated: Sep 6, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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Published on: May 10, 2024

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CRISPR-Cas9-Based Technology for Studying Enteric Virus Infection.

Junki Hirano1, Kosuke Murakami1, Tsuyoshi Hayashi1

  • 1Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.

Frontiers in Genome Editing
|June 27, 2022
PubMed
Summary

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system is revolutionizing enteric virus research. This technology helps identify host factors crucial for viral replication and pathogenicity, paving the way for new antiviral strategies.

Keywords:
CRISPR-Cas9enteric virusgene knockoutgenome wide screenhost factorsnorovirusrotavirus

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Enteric viruses cause significant human illnesses via fecal-oral transmission.
  • Understanding host-virus interactions is key to developing antiviral therapies.
  • Existing research lacks comprehensive identification of host factors involved in enteric virus replication.

Purpose of the Study:

  • To review the application of CRISPR-Cas9 technology in enteric virus research.
  • To highlight the identification of novel host factors influencing enteric virus infection.
  • To discuss the implications for developing antiviral strategies.

Main Methods:

  • Utilizing the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system for genome-wide screening.
  • Employing unbiased screening to identify host factors.
  • Reviewing existing literature on CRISPR-Cas9 applications in virology.

Main Results:

  • CRISPR-Cas9 has successfully identified previously unrecognized host factors for enteric virus infection.
  • These identified factors play roles in viral replication and pathogenicity.
  • The technology enables a deeper understanding of molecular mechanisms.

Conclusions:

  • CRISPR-Cas9 is a powerful tool for advancing enteric virus research.
  • Identification of host factors using this system is crucial for therapeutic development.
  • Further research using CRISPR-Cas9 will enhance antiviral strategies against enteric viruses.