Genetic Relationship and Evolution Analysis among Malus Mill Plant Populations Based on SCoT Molecular Markers

Malus Mill genotype is highly heterozygous, and many theoretical problems such as genetic relationship and evolution process among germplasm are difficult to be solved by traditional analysis methods. The development of SCoT(start codon targeted polymorphism) molecular markers suitable for apples is of great significance for studying the origin, evolution, genetic relationship and genetic diversity of Malus Mill germplasm resources. In this paper, the genetic relationship and evolution of 15 materials were analyzed by SCoT molecular marker. The results showed that the gene differentiation coefficient values of four Malus Mill plants at the species level were 0.423, 0.439, 0.428 and 0.460, respectively, which indicated that there was obvious genetic differentiation among the populations of these four Malus Mill plants, but there were some differences among the populations of different Malus Mill plants. The gene differentiation coefficient of coextensive populations with different geographical distribution varied from 0.177 to 0.086 (average 0.138), which indicated that the genetic similarity of species in coextensive composite populations was high and there was a close genetic relationship among species. This indicates that SCoT molecular markers can be effectively used in the analysis of intraspecific genetic relationship and identification of intraspecific strains of Malus Mill plants.