Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

What is Genetic Engineering?

What is Genetic Engineering?

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multi-organ acute and delayed effects following exposure to neutron radiation.

iScience·2026

Same author

Pirfenidone Inhibits Inflammatory Mediators in Human Cornea <i>In Vitro</i>.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2026

Same author

Retinopathy of Prematurity (ROP): Are We There Yet?

Medicina (Kaunas, Lithuania)·2026

Same author

Endothelial-perivascular mural partitioning and multicellular signaling in injury-associated corneal neovascularization.

Experimental eye research·2026

Same author

Development and partial characterization of fused human endostatin.

Protein expression and purification·2026

Same author

Myogenic Differentiation (MyoD) Gene Expression in Cornea and Role in Corneal Myofibroblast Dedifferentiation.

Investigative ophthalmology & visual science·2026

Search research articles

Related Experiment Video

Updated: Sep 6, 2025

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Gene editing for corneal disease management.

Sudhanshu P Raikwar¹, Apoorva S Raikwar², Shyam S Chaurasia¹

¹Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, United States.

World Journal of Translational Medicine

|June 27, 2022

Summary

Gene editing technologies like CRISPR offer precise genetic modifications for corneal disorders. This approach promises to revolutionize diagnostics and treatments, potentially curing blindness.

Keywords:

Adeno-associated virus Clustered Regularly Interspaced Short Palindromic Repeat Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9 Cornea Double strand breaks Gene editing Gene targeting Homologous recombination Homology directed repair Indels Lentiviral vector Protospacer-adjacent motif Transcription activator like effector nucleases Zinc finger nucleases sgRNA

More Related Videos

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Published on: April 24, 2020

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Related Experiment Videos

Last Updated: Sep 6, 2025

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Published on: April 24, 2020

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Ophthalmology
Molecular Biology
Biotechnology

Background:

Gene editing is a rapidly advancing technology for precise genomic modifications.
Novel tools like CRISPR, zinc finger nucleases, and TALENs enable high-throughput genome engineering.
These advancements are crucial for understanding and treating complex eye diseases.

Purpose of the Study:

To review the latest developments in genome editing for corneal diseases.
To discuss current challenges and future prospects of gene-based personalized medicine for ocular conditions.
To highlight the potential of gene editing to revolutionize blindness treatment.

Main Methods:

Review of recent scientific literature on gene editing technologies.
Analysis of applications in corneal disease research and therapy.
Discussion of CRISPR-associated systems, zinc finger nucleases, and TALENs.

Main Results:

Gene editing enables precise genetic modifications for long-term relief from corneal disorders.
It facilitates the deciphering of disease mechanisms and the development of next-generation therapies.
Potential applications include gene therapy, regenerative medicine, and personalized medicine for ocular diseases.

Conclusions:

Gene editing is poised to transform the diagnosis and treatment of corneal diseases.
This technology holds significant promise for curing blindness through personalized gene-based medicine.
Continued research and development are essential to overcome challenges and realize the full potential of gene editing in ophthalmology.