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Attenuated Duck Hepatitis A Virus Infection Is Associated With High mRNA Maintenance in Duckling Liver via m6A
Liping Wu1, Weili Quan2, Yi Zhang2
1Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Frontiers in Immunology
|June 27, 2022
Summary
Duck hepatitis A virus (DHAV) infection impacts host translation. This study reveals DHAV virulence influences N6-methyladenosine (m6A) modification levels in duckling livers, affecting mRNA expression and immune responses.
Area of Science:
- Virology
- Molecular Biology
- Epigenetics
Background:
- Viral infections modulate host translation.
- Duck hepatitis A virus (DHAV) infection inhibits cellular protein synthesis.
- N6-methyladenosine (m6A) modification is crucial for RNA regulation.
Purpose of the Study:
- To investigate the impact of DHAV virulence on cellular m6A modification.
- To understand the relationship between m6A levels and mRNA expression in DHAV-infected ducklings.
Main Methods:
- m6A antibody-based immunoprecipitation
- m6A-seq
- LC-MS/MS
- RNA-seq
- Gene Ontology (GO) analysis
Main Results:
- m6A-modified mRNA is present in both virulent and attenuated DHAV-infected duckling livers.
- m6A levels are significantly higher in attenuated DHAV-infected livers compared to virulent ones.
- A positive correlation exists between m6A levels and mRNA expression.
- Genes with altered m6A levels are involved in oxidation-reduction and antiviral immune responses.
Conclusions:
- DHAV virulence-dependently regulates m6A modification.
- m6A modification and mRNA expression are coordinated in DHAV-infected duckling livers.
- These findings highlight a role for m6A in the host-pathogen interaction during DHAV infection.
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