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Related Concept Videos

Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
126
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

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Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
137

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Development and Application of Two Inducible Expression Systems for Streptococcus suis.

Liangsheng Zhang1, Wenjin Zou1, Minghui Ni1

  • 1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural Universitygrid.35155.37, Wuhan, China.

Microbiology Spectrum
|June 27, 2022
PubMed
Summary

Researchers developed two inducible expression systems for Streptococcus suis, enabling controlled gene studies. These tools aid in understanding bacterial growth, division, and identifying new antimicrobial targets.

Keywords:
Streptococcus suisconditional gene knockoutgenetic toolinducible expression systemsubcellular localization

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Streptococcus suis is a significant zoonotic pathogen impacting swine and human health.
  • Understanding S. suis growth and cell division mechanisms is crucial for public health and the pig industry.
  • Existing genetic tools for S. suis lack precise control over gene expression, hindering fundamental research.

Purpose of the Study:

  • To develop novel, tightly regulated inducible expression systems for Streptococcus suis.
  • To facilitate functional studies of essential genes and protein localization in S. suis.
  • To provide valuable genetic tools for investigating S. suis physiology and identifying antimicrobial targets.

Main Methods:

  • Identification and characterization of three strong constitutive promoters (Pg, Pt, Pe) in S. suis.
  • Construction of anhydrotetracycline (ATc)-inducible and isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible expression systems.
  • Optimization of operator sequence insertion sites for precise gene regulation.
  • Validation of systems using reporter gene expression, subcellular localization of FtsZ, and conditional knockout of the essential glmS gene.

Main Results:

  • Successfully established ATc-inducible and IPTG-inducible expression systems in S. suis.
  • Demonstrated inducer-concentration and time-dependent reporter gene expression.
  • Confirmed correct midcell localization of the FtsZ protein using the developed systems.
  • Constructed a conditional glmS knockout strain, validating the systems for essential gene studies.

Conclusions:

  • The developed inducible expression systems offer unprecedented control over gene expression in S. suis.
  • These systems are effective for studying protein localization and essential gene functions.
  • The new genetic tools will significantly advance functional genomics and the search for antimicrobial targets in S. suis.