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Updated: Sep 6, 2025

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds
Published on: August 8, 2025
Quorum sensing inhibitory potential of vaccenic acid against Chromobacterium violaceum and methicillin-resistant
Vijayakumar Karuppiah1,2, Muhilvannan Seralathan3
1Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, Cuddalore, Tamil Nadu, 608 502, India. Vijaybiotech92@gmail.com.
Abstract:
Quorum sensing (QS) is a potential target for inhibiting bacterial antibiotic resistance and associated pathogenicity. The present study aimed to investigate vaccenic acid anti-QS and antibiofilm potential against Chromobacterium violaceum and methicillin-resistant Staphylococcus aureus (MRSA). In the broth microdilution method, we determined the minimum inhibitory concentration (MIC) of vaccenic acid against C. violaceum and MRSA. Then, we determined the vaccenic acid anti-QS potential against C. violaceum via a violacein inhibition assay. Vaccenic acid at a sub-MIC concentration significantly inhibited violacein pigment production. Vaccenic acid also inhibits C. violaceum and MRSA biofilm formation at sub-MIC concentrations. The effect of vaccenic acid antivirulence potential was evaluated by phenotypic virulence assays. The results showed that vaccenic acid at a sub-MIC concentration significantly inhibited the virulence production of C. violaceum (chitinase and motility) and MRSA (hemolysin and staphyloxanthin production). Quantitative PCR analysis revealed the downregulation of QS associated genes upon vaccenic acid treatment. This resulted in the downregulation of genes involved in QS mechanisms such as cviI, cviR, and SarA and pigment production such as vioB and crtM. The results of the present study suggest that vaccenic acid is a promising agent to combat C. violaceum and MRSA.
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