Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts

Area of Science:

• Dermatology
• Biochemistry
• Cell Biology

Background:

• Oxidative damage is a primary driver of human skin aging.
• Inotodiol, a lanostane triterpenoid, is known for antiviral, anticancer, anti-inflammatory, and antiallergic properties.
• The effect of inotodiol on oxidative stress-induced skin aging remains unexplored.

Purpose of the Study:

• To investigate the potential of inotodiol in preventing oxidative stress-induced human skin aging.
• To elucidate the molecular mechanisms underlying inotodiol's effects on human dermal fibroblasts.

Main Methods:

• Human dermal fibroblast cells were stimulated with hydrogen peroxide to induce aging.
• The expression of mitogen-activated protein kinase (MAPK) and NADPH Oxidase 5 (NOX5) was analyzed.
• Levels of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), nitric oxide (NO), reactive oxygen species (ROS), cyclooxygenase-2 (COX-2), and cytokines (IL-1β, IL-6, TNF-α) were measured.

Main Results:

• Inotodiol significantly inhibited the expression of MAPK and NOX5.
• Inotodiol reduced the activation of NF-κB signaling.
• Inotodiol decreased levels of NO, ROS, COX-2, and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α).

Conclusions:

• Inotodiol protects human dermal fibroblasts against oxidative stress-induced aging.
• The protective effects are mediated by the inhibition of MAPK-NOX5 and NF-κB pathways.
• Inotodiol shows potential as a natural ingredient for anti-aging cosmetic products.