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Updated: Sep 6, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

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miR-320a promotes p53-dependent apoptosis of prostate cancer cells by negatively regulating TP73-AS1 invitro

Esra Bozgeyik1, Ahmet Arslan2, Ebru Temiz3

  • 1Department of Medical Services and Techniques, Vocational School of Health Services, Adiyaman University, Adiyaman, Turkey.

Biochemical and Biophysical Research Communications
|June 27, 2022
PubMed

Insights

MicroRNA-320a suppresses prostate cancer growth by targeting TP73 antisense RNA 1 (TP73-AS1). Overexpression of miR-320a induces apoptosis and inhibits cell proliferation by downregulating TP73-AS1.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • TP73 antisense RNA 1 (TP73-AS1) is an oncogenic long non-coding RNA implicated in various cancers.
  • Mechanisms regulating TP73-AS1 in prostate cancer, particularly post-transcriptional regulation by microRNAs (miRNAs), are not well understood.

Purpose of the Study:

  • To identify miRNAs regulating TP73-AS1 in prostate cancer.
  • To investigate the impact of these miRNAs on prostate cancer cell proliferation and apoptosis.

Main Methods:

  • Prostate cancer cell lines were utilized to study miRNA regulation of TP73-AS1.
  • Overexpression of specific miRNAs (miR-200a, miR-320a) and assessment of TP73-AS1 levels were performed.
  • Cell proliferation, colony formation, cell migration, cell cycle, and apoptosis assays were conducted.

Main Results:

  • Overexpression of miR-200a and miR-320a suppressed prostate cancer cell colony formation and migration, while inducing cell cycle arrest and apoptosis.
  • miR-200a and miR-320a were upregulated in prostate cancer cells with suppressed TP73-AS1.
  • TP73-AS1 was downregulated upon miR-200a and miR-320a overexpression; miR-320a induced p53-dependent apoptosis.

Conclusions:

  • miR-320a acts as a tumor suppressor in prostate cancer by negatively regulating the oncogenic TP73-AS1 lncRNA.
  • miR-320a induces p53-dependent apoptosis, offering a potential therapeutic target for prostate cancer.
Keywords:
CancerProstate cancerTP73-AS1miR-200amiR-320ap53

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