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Related Experiment Video

Updated: Sep 6, 2025

Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce Lactuca sativa Germplasm Collections

Semi-High Throughput Screening for Potential Drought-tolerance in Lettuce Lactuca sativa Germplasm Collections

Published on: April 17, 2015

Selecting putative drought-tolerance markers in two contrasting soybeans.

Laila Toum¹, Lucia Sandra Perez-Borroto^2,3, Andrea Natalia Peña-Malavera¹

¹Instituto de Tecnología Agroindustrial del Noroeste Argentino, Estación Experimental Agroindustrial Obispo Colombres- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), William Cross 3150, Las Talitas, Tucumán, Argentina.

Scientific Reports

|June 27, 2022

Summary

Identifying drought-tolerant soybean varieties is crucial for climate-smart agriculture. This study highlights pubescence, stomatal density, and canopy temperature depression as key early selection markers for yield under water scarcity.

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Last Updated: Sep 6, 2025

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Published on: April 17, 2015

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Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes

Published on: March 28, 2025

Area of Science:

Agricultural Science
Plant Physiology
Genetics

Background:

Climate-smart breeding requires identifying high-yield soybean genotypes resilient to low water availability.
Phenotyping bottlenecks hinder the selection of cost-efficient markers for drought tolerance and yield stabilization.

Purpose of the Study:

To understand soybean stress physiology under water deficit.
To identify and validate drought-tolerance and yield-stabilization traits as potential breeding markers.

Main Methods:

Massive analysis of cDNA ends (MACE)-transcriptomic analysis to explore molecular differences between genotypes.
In-depth phenotyping of 22 stress-response, growth, and water-use markers under mild water deficit.
Correlation analysis of markers across phenological stages and with final yield.

Main Results:

Significant transcriptional differences were observed between drought-tolerant (MUNASQA) and susceptible (TJ2049) soybean genotypes.
Several phenotyping markers demonstrated high consistency across vegetative (V3) and reproductive (R5) stages.
Pubescence, stomatal density, and canopy temperature depression were identified as promising, cost-feasible markers for early selection.

Conclusions:

The study validates a phenotyping methodology for early selection of drought-tolerant soybean genotypes.
Selected markers (pubescence, stomatal density, canopy temperature depression) offer practical applications for soybean breeding programs aiming for drought resilience.