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Decrease of Membrane B7-H5 on CD14+ Cells in Severe Acute Pancreatitis Is Related to RANSON Scores and APACHE II

Ruoxin Xu1, Siming Xie1, Ju Gong2

  • 1Department of Critical Care Medicine, The First Affiliated Hospital of Suzhou University, No.188 Shizi Street, Suzhou, 215000, Jiangsu, China.

Digestive Diseases and Sciences
|June 27, 2022
PubMed
Abstract

Insights

B7-H5 levels change in severe acute pancreatitis (SAP). Decreased membrane B7-H5 (mB7-H5) on immune cells correlates with SAP severity and may indicate a poor prognosis.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • B7-H5 is a key immune response ligand.
  • Its role as an early indicator in acute pancreatitis (AP) is not well understood.

Purpose of the Study:

  • To investigate the role of B7-H5 in severe acute pancreatitis (SAP).

Main Methods:

  • Measured soluble B7-H5 (sB7-H5) in plasma and membrane B7-H5 (mB7-H5) on CD14+ cells in SAP patients and healthy donors.
  • Stimulated peripheral blood mononuclear cells (PBMCs) with SAP patient serum, LPS, TNF-α, or IFN-γ to assess B7-H5 expression changes.
  • Correlated mB7-H5 levels with clinical features of SAP patients.

Main Results:

  • SAP patients showed increased sB7-H5 and decreased mB7-H5 on CD14+ cells.
  • SAP patient serum, LPS, TNF-α, or IFN-γ induced similar B7-H5 expression changes in vitro.
  • Decreased mB7-H5 levels correlated negatively with hematocrit, urea nitrogen, creatinine, lactic acid, RANSON, and APACHE II scores.

Conclusions:

  • Altered B7-H5 expression patterns are implicated in the immune response during SAP.
  • A decrease in mB7-H5 due to innate immune activation may be linked to a poorer prognosis in SAP patients.
Keywords:
B7-H5IFN-γLPSSevere acute pancreatitisTNF-α

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