Follicular-fluid proteomics during equine follicle development
Ghassan M Ishak1,2, Jean M Feugang3, Olga Pechanova4
1Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Molecular Reproduction and Development
|June 28, 2022
Summary
This study profiles equine follicular fluid proteins during follicle development. Key proteins, like those in the complement system, are vital for dominant follicle selection and ovulation, aiding oocyte quality assessment.
Area of Science:
- Reproductive Biology
- Proteomics
- Biochemistry
Background:
- Follicular fluid (FF) composition impacts oocyte development and function.
- Comprehensive proteomic profiling of equine FF across follicle development stages is lacking.
Purpose of the Study:
- To comprehensively profile the equine FF proteome during key stages of follicle selection, development, and ovulation.
- To identify proteins and pathways involved in these processes for potential biomarker discovery.
Main Methods:
- Shotgun proteomics approach was employed to analyze equine FF.
- Proteins were harvested from follicles at distinct developmental stages: predeviation, deviation, postdeviation, preovulatory, and impending ovulation.
- Bioinformatics analyses were used for functional interpretation of detected proteins.
Main Results:
- A total of 294 proteins were identified in equine FF.
- Proteins of the complement system, including properdin, showed higher expression during deviation and ovulation.
- The 'complement and coagulation cascades' pathway was predominant across all developmental stages.
Conclusions:
- The study provides an extensive proteomic map of equine FF during folliculogenesis and ovulation.
- Complement system proteins play a significant role in dominant follicle selection and ovulation.
- Findings offer insights into ovarian function, ovulatory disorders, and potential biomarkers for oocyte quality.
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