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Lipoprotein uptake by neuronal growth cones in vitro
Science (New York, N.Y.)
|May 22, 1987
Summary
Apolipoprotein E delivers lipids to regenerating nerve cells via lipoprotein receptors. This process supports neurite growth and membrane biosynthesis during peripheral nerve repair.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Macrophages in injured peripheral nerves secrete apolipoprotein E.
- Apolipoprotein E may redistribute lipids, including cholesterol, to regenerating axons.
Purpose of the Study:
- To investigate if sprouting neurites and their growth cones have lipoprotein receptors.
- To determine the role of apolipoprotein E in lipid delivery to regenerating neurons.
Main Methods:
- Used fluorescently labeled apolipoprotein E-lipid particles and lipoproteins.
- Utilized Pheochromocytoma (PC12) cells as an in vitro model for neurite growth.
- Observed internalization of labeled particles by neurites and growth cones.
Main Results:
- Neurites and growth cones internalized apolipoprotein E-containing particles and lipoproteins.
- Accumulation in growth cones ruled out transport from cell bodies.
- Evidence suggests receptor-mediated uptake via apolipoprotein B,E(LDL) receptors.
Conclusions:
- Apolipoprotein E facilitates lipid uptake by regenerating peripheral nerve neurites.
- Lipoprotein receptors on growth cones are crucial for delivering lipids for membrane biosynthesis.
- This mechanism is vital for peripheral nerve regeneration.