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Updated: Sep 6, 2025
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
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An Fe-based single-atom nanozyme with multi-enzyme activity for parallel catalytic therapy via a cascade reaction
Rui Niu1,2, Yang Liu1,2, Yinghui Wang1,2
1State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry (CIAC), Chinese Academy of Sciences, Changchun, 130022, China. yhwang@ciac.ac.cn.
Chemical Communications (Cambridge, England)
|June 28, 2022
Summary
We developed iron-based single-atom nanozymes that efficiently produce reactive oxygen species (ROS) in tumors. These nanozymes also offer photothermal therapy (PTT) benefits, enhancing cancer treatment effectiveness.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cancer Therapeutics
Background:
- The tumor microenvironment (TME) presents unique challenges for cancer treatment.
- Developing novel nanomaterials with multiple therapeutic functions is crucial for improving treatment efficacy.
Purpose of the Study:
- To fabricate and characterize Fe-based single-atom nanozymes (FeSA-HNCSB) for cancer therapy.
- To evaluate the ROS generation and enzyme-mimicking activities of FeSA-HNCSB in the TME.
- To assess the potential of FeSA-HNCSB for combined catalytic therapy and photothermal therapy (PTT).
Main Methods:
- Synthesis of Fe-based single-atom nanozymes by embedding Fe atoms into N-doped hollow carbon spheres.
- Assessment of ROS generation capabilities in simulated TME conditions.
- Evaluation of catalase-like, oxidase-like, and peroxidase-like enzyme activities.
- Measurement of photothermal conversion efficiency in the near-infrared (NIR) region.
Main Results:
- FeSA-HNCSB nanozymes efficiently catalyze ROS generation in the TME.
- Nanozymes demonstrated multiple enzyme-mimicking activities.
- FeSA-HNCSB exhibited significant photothermal conversion efficiency in the NIR region.
- The combined therapy approach showed potential for enhanced tumor treatment.
Conclusions:
- Fe-based single-atom nanozymes (FeSA-HNCSB) are effective in generating ROS and possess multiple enzyme-mimicking activities.
- FeSA-HNCSB show promise for integrated photothermal and catalytic cancer therapy.
- This study presents a novel strategy for improving tumor treatment through parallel catalytic therapy and PTT.
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